DetoxFungiV1, Main, Exploration, bibRecord, 001458

Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells.

Identifieur interne : 001458 ( Main/Exploration ); précédent : 001457; suivant : 001459

Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells.

Auteurs : Andreja Rajkovic [Belgique] ; Charlotte Grootaert [Belgique] ; Ana Butorac [Croatie] ; Tatiana Cucu [Belgique] ; Bruno De Meulenaer [Belgique] ; John Van Camp [Belgique] ; Marc Bracke [Belgique] ; Mieke Uyttendaele [Belgique] ; Višnja Ba Un-Družina [Croatie] ; Mario Cindri [Croatie]

Source :

Toxins [ 2072-6651 ] ; 2014.

RBID : pubmed:25093386

Descripteurs français

KwdFr :
- Acide lactique (métabolisme), Animaux (MeSH), Bacillus cereus (MeSH), Cellules Caco-2 (MeSH), Depsipeptides (toxicité), Entérocytes (effets des médicaments et des substances chimiques), Entérocytes (métabolisme), Humains (MeSH), Mobilité des spermatozoïdes (effets des médicaments et des substances chimiques), Mâle (MeSH), Protéomique (MeSH), Survie cellulaire (effets des médicaments et des substances chimiques), Sus scrofa (MeSH), Émétiques (toxicité).
MESH :
- effets des médicaments et des substances chimiques : Entérocytes, Mobilité des spermatozoïdes, Survie cellulaire.
- métabolisme : Acide lactique, Entérocytes.
- toxicité : Depsipeptides, Émétiques.
- Animaux, Bacillus cereus, Cellules Caco-2, Humains, Mâle, Protéomique, Sus scrofa.

English descriptors

KwdEn :
- Animals (MeSH), Bacillus cereus (MeSH), Caco-2 Cells (MeSH), Cell Survival (drug effects), Depsipeptides (toxicity), Emetics (toxicity), Enterocytes (drug effects), Enterocytes (metabolism), Humans (MeSH), Lactic Acid (metabolism), Male (MeSH), Proteomics (MeSH), Sperm Motility (drug effects), Sus scrofa (MeSH).
MESH :
- chemical , metabolism : Lactic Acid.
- chemical , toxicity : Depsipeptides, Emetics.
- drug effects : Cell Survival, Enterocytes, Sperm Motility.
- metabolism : Enterocytes.
- Animals, Bacillus cereus, Caco-2 Cells, Humans, Male, Proteomics, Sus scrofa.

Abstract

Cereulide (CER) intoxication occurs at relatively high doses of 8 µg/kg body weight. Recent research demonstrated a wide prevalence of low concentrations of CER in rice and pasta dishes. However, the impact of exposure to low doses of CER has not been studied before. In this research, we investigated the effect of low concentrations of CER on the behavior of intestinal cells using the Caco-2 cell line. The MTT (mitochondrial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and the SRB (sulforhodamine B) reactions were used to measure the mitochondrial activity and cellular protein content, respectively. Both assays showed that differentiated Caco-2 cells were sensitive to low concentrations of CER (in a MTT reaction of 1 ng/mL after three days of treatment; in an SRB reaction of 0.125 ng/mL after three days of treatment). Cell counts revealed that cells were released from the differentiated monolayer at 0.5 ng/mL of CER. Additionally, 0.5 and 2 ng/mL of CER increased the lactate presence in the cell culture medium. Proteomic data showed that CER at a concentration of 1 ng/mL led to a significant decrease in energy managing and H2O2 detoxification proteins and to an increase in cell death markers. This is amongst the first reports to describe the influence of sub-emetic concentrations of CER on a differentiated intestinal monolayer model showing that low doses may induce an altered enterocyte metabolism and membrane integrity.

DOI: 10.3390/toxins6082270
PubMed: 25093386
PubMed Central: PMC4147582

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 001473
to stream Main, to step Curation: 001473

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells.</title>
<author><name sortKey="Rajkovic, Andreja" sort="Rajkovic, Andreja" uniqKey="Rajkovic A" first="Andreja" last="Rajkovic">Andreja Rajkovic</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000, Belgium. Andreja.Rajkovic@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Grootaert, Charlotte" sort="Grootaert, Charlotte" uniqKey="Grootaert C" first="Charlotte" last="Grootaert">Charlotte Grootaert</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. Charlotte.Grootaert@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Butorac, Ana" sort="Butorac, Ana" uniqKey="Butorac A" first="Ana" last="Butorac">Ana Butorac</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000, Croatia. abutorac@pbf.hr.</nlm:affiliation>
<country xml:lang="fr">Croatie</country>
<wicri:regionArea>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000</wicri:regionArea>
<wicri:noRegion>Zagreb HR-10000</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cucu, Tatiana" sort="Cucu, Tatiana" uniqKey="Cucu T" first="Tatiana" last="Cucu">Tatiana Cucu</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. Tatiana.Cucu@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="De Meulenaer, Bruno" sort="De Meulenaer, Bruno" uniqKey="De Meulenaer B" first="Bruno" last="De Meulenaer">Bruno De Meulenaer</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. bruno.demeulenaer@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Van Camp, John" sort="Van Camp, John" uniqKey="Van Camp J" first="John" last="Van Camp">John Van Camp</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. John.VanCamp@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bracke, Marc" sort="Bracke, Marc" uniqKey="Bracke M" first="Marc" last="Bracke">Marc Bracke</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Experimental Cancer Research, University Hospital Ghent, Ghent B-9000, Belgium. Marc1.Bracke@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Experimental Cancer Research, University Hospital Ghent, Ghent B-9000</wicri:regionArea>
<wicri:noRegion>Ghent B-9000</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Uyttendaele, Mieke" sort="Uyttendaele, Mieke" uniqKey="Uyttendaele M" first="Mieke" last="Uyttendaele">Mieke Uyttendaele</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000, Belgium. Mieke.Uyttendaele@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ba Un Druzina, Visnja" sort="Ba Un Druzina, Visnja" uniqKey="Ba Un Druzina V" first="Višnja" last="Ba Un-Družina">Višnja Ba Un-Družina</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000, Croatia.</nlm:affiliation>
<country xml:lang="fr">Croatie</country>
<wicri:regionArea>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000</wicri:regionArea>
<wicri:noRegion>Zagreb HR-10000</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cindri, Mario" sort="Cindri, Mario" uniqKey="Cindri M" first="Mario" last="Cindri">Mario Cindri</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory for System Biomedicine and Centre for Proteomics and Mass Spectrometry, "Ruđer Bošković" Institute, Zagreb HR-10000, Croatia. mcindric@irb.hr.</nlm:affiliation>
<country xml:lang="fr">Croatie</country>
<wicri:regionArea>Laboratory for System Biomedicine and Centre for Proteomics and Mass Spectrometry, "Ruđer Bošković" Institute, Zagreb HR-10000</wicri:regionArea>
<wicri:noRegion>Zagreb HR-10000</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2014">2014</date>
<idno type="RBID">pubmed:25093386</idno>
<idno type="pmid">25093386</idno>
<idno type="doi">10.3390/toxins6082270</idno>
<idno type="pmc">PMC4147582</idno>
<idno type="wicri:Area/Main/Corpus">001473</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001473</idno>
<idno type="wicri:Area/Main/Curation">001473</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001473</idno>
<idno type="wicri:Area/Main/Exploration">001473</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells.</title>
<author><name sortKey="Rajkovic, Andreja" sort="Rajkovic, Andreja" uniqKey="Rajkovic A" first="Andreja" last="Rajkovic">Andreja Rajkovic</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000, Belgium. Andreja.Rajkovic@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Grootaert, Charlotte" sort="Grootaert, Charlotte" uniqKey="Grootaert C" first="Charlotte" last="Grootaert">Charlotte Grootaert</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. Charlotte.Grootaert@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Butorac, Ana" sort="Butorac, Ana" uniqKey="Butorac A" first="Ana" last="Butorac">Ana Butorac</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000, Croatia. abutorac@pbf.hr.</nlm:affiliation>
<country xml:lang="fr">Croatie</country>
<wicri:regionArea>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000</wicri:regionArea>
<wicri:noRegion>Zagreb HR-10000</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cucu, Tatiana" sort="Cucu, Tatiana" uniqKey="Cucu T" first="Tatiana" last="Cucu">Tatiana Cucu</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. Tatiana.Cucu@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="De Meulenaer, Bruno" sort="De Meulenaer, Bruno" uniqKey="De Meulenaer B" first="Bruno" last="De Meulenaer">Bruno De Meulenaer</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. bruno.demeulenaer@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Van Camp, John" sort="Van Camp, John" uniqKey="Van Camp J" first="John" last="Van Camp">John Van Camp</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. John.VanCamp@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bracke, Marc" sort="Bracke, Marc" uniqKey="Bracke M" first="Marc" last="Bracke">Marc Bracke</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Experimental Cancer Research, University Hospital Ghent, Ghent B-9000, Belgium. Marc1.Bracke@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Experimental Cancer Research, University Hospital Ghent, Ghent B-9000</wicri:regionArea>
<wicri:noRegion>Ghent B-9000</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Uyttendaele, Mieke" sort="Uyttendaele, Mieke" uniqKey="Uyttendaele M" first="Mieke" last="Uyttendaele">Mieke Uyttendaele</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000, Belgium. Mieke.Uyttendaele@UGent.be.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000</wicri:regionArea>
<orgName type="university">Université de Gand</orgName>
<placeName><settlement type="city">Gand</settlement>
<region>Région flamande</region>
<region type="district" nuts="2">Province de Flandre-Orientale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ba Un Druzina, Visnja" sort="Ba Un Druzina, Visnja" uniqKey="Ba Un Druzina V" first="Višnja" last="Ba Un-Družina">Višnja Ba Un-Družina</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000, Croatia.</nlm:affiliation>
<country xml:lang="fr">Croatie</country>
<wicri:regionArea>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000</wicri:regionArea>
<wicri:noRegion>Zagreb HR-10000</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cindri, Mario" sort="Cindri, Mario" uniqKey="Cindri M" first="Mario" last="Cindri">Mario Cindri</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory for System Biomedicine and Centre for Proteomics and Mass Spectrometry, "Ruđer Bošković" Institute, Zagreb HR-10000, Croatia. mcindric@irb.hr.</nlm:affiliation>
<country xml:lang="fr">Croatie</country>
<wicri:regionArea>Laboratory for System Biomedicine and Centre for Proteomics and Mass Spectrometry, "Ruđer Bošković" Institute, Zagreb HR-10000</wicri:regionArea>
<wicri:noRegion>Zagreb HR-10000</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Toxins</title>
<idno type="eISSN">2072-6651</idno>
<imprint><date when="2014" type="published">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Bacillus cereus (MeSH)</term>
<term>Caco-2 Cells (MeSH)</term>
<term>Cell Survival (drug effects)</term>
<term>Depsipeptides (toxicity)</term>
<term>Emetics (toxicity)</term>
<term>Enterocytes (drug effects)</term>
<term>Enterocytes (metabolism)</term>
<term>Humans (MeSH)</term>
<term>Lactic Acid (metabolism)</term>
<term>Male (MeSH)</term>
<term>Proteomics (MeSH)</term>
<term>Sperm Motility (drug effects)</term>
<term>Sus scrofa (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acide lactique (métabolisme)</term>
<term>Animaux (MeSH)</term>
<term>Bacillus cereus (MeSH)</term>
<term>Cellules Caco-2 (MeSH)</term>
<term>Depsipeptides (toxicité)</term>
<term>Entérocytes (effets des médicaments et des substances chimiques)</term>
<term>Entérocytes (métabolisme)</term>
<term>Humains (MeSH)</term>
<term>Mobilité des spermatozoïdes (effets des médicaments et des substances chimiques)</term>
<term>Mâle (MeSH)</term>
<term>Protéomique (MeSH)</term>
<term>Survie cellulaire (effets des médicaments et des substances chimiques)</term>
<term>Sus scrofa (MeSH)</term>
<term>Émétiques (toxicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lactic Acid</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Depsipeptides</term>
<term>Emetics</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Survival</term>
<term>Enterocytes</term>
<term>Sperm Motility</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Entérocytes</term>
<term>Mobilité des spermatozoïdes</term>
<term>Survie cellulaire</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Enterocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide lactique</term>
<term>Entérocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Depsipeptides</term>
<term>Émétiques</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Bacillus cereus</term>
<term>Caco-2 Cells</term>
<term>Humans</term>
<term>Male</term>
<term>Proteomics</term>
<term>Sus scrofa</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Bacillus cereus</term>
<term>Cellules Caco-2</term>
<term>Humains</term>
<term>Mâle</term>
<term>Protéomique</term>
<term>Sus scrofa</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Cereulide (CER) intoxication occurs at relatively high doses of 8 µg/kg body weight. Recent research demonstrated a wide prevalence of low concentrations of CER in rice and pasta dishes. However, the impact of exposure to low doses of CER has not been studied before. In this research, we investigated the effect of low concentrations of CER on the behavior of intestinal cells using the Caco-2 cell line. The MTT (mitochondrial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and the SRB (sulforhodamine B) reactions were used to measure the mitochondrial activity and cellular protein content, respectively. Both assays showed that differentiated Caco-2 cells were sensitive to low concentrations of CER (in a MTT reaction of 1 ng/mL after three days of treatment; in an SRB reaction of 0.125 ng/mL after three days of treatment). Cell counts revealed that cells were released from the differentiated monolayer at 0.5 ng/mL of CER. Additionally, 0.5 and 2 ng/mL of CER increased the lactate presence in the cell culture medium. Proteomic data showed that CER at a concentration of 1 ng/mL led to a significant decrease in energy managing and H2O2 detoxification proteins and to an increase in cell death markers. This is amongst the first reports to describe the influence of sub-emetic concentrations of CER on a differentiated intestinal monolayer model showing that low doses may induce an altered enterocyte metabolism and membrane integrity. </div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25093386</PMID>
<DateCompleted><Year>2015</Year>
<Month>03</Month>
<Day>31</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2072-6651</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>6</Volume>
<Issue>8</Issue>
<PubDate><Year>2014</Year>
<Month>Aug</Month>
<Day>04</Day>
</PubDate>
</JournalIssue>
<Title>Toxins</Title>
<ISOAbbreviation>Toxins (Basel)</ISOAbbreviation>
</Journal>
<ArticleTitle>Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells.</ArticleTitle>
<Pagination><MedlinePgn>2270-90</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.3390/toxins6082270</ELocationID>
<Abstract><AbstractText>Cereulide (CER) intoxication occurs at relatively high doses of 8 µg/kg body weight. Recent research demonstrated a wide prevalence of low concentrations of CER in rice and pasta dishes. However, the impact of exposure to low doses of CER has not been studied before. In this research, we investigated the effect of low concentrations of CER on the behavior of intestinal cells using the Caco-2 cell line. The MTT (mitochondrial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and the SRB (sulforhodamine B) reactions were used to measure the mitochondrial activity and cellular protein content, respectively. Both assays showed that differentiated Caco-2 cells were sensitive to low concentrations of CER (in a MTT reaction of 1 ng/mL after three days of treatment; in an SRB reaction of 0.125 ng/mL after three days of treatment). Cell counts revealed that cells were released from the differentiated monolayer at 0.5 ng/mL of CER. Additionally, 0.5 and 2 ng/mL of CER increased the lactate presence in the cell culture medium. Proteomic data showed that CER at a concentration of 1 ng/mL led to a significant decrease in energy managing and H2O2 detoxification proteins and to an increase in cell death markers. This is amongst the first reports to describe the influence of sub-emetic concentrations of CER on a differentiated intestinal monolayer model showing that low doses may induce an altered enterocyte metabolism and membrane integrity. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rajkovic</LastName>
<ForeName>Andreja</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000, Belgium. Andreja.Rajkovic@UGent.be.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Grootaert</LastName>
<ForeName>Charlotte</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. Charlotte.Grootaert@UGent.be.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Butorac</LastName>
<ForeName>Ana</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000, Croatia. abutorac@pbf.hr.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cucu</LastName>
<ForeName>Tatiana</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. Tatiana.Cucu@UGent.be.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>De Meulenaer</LastName>
<ForeName>Bruno</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. bruno.demeulenaer@UGent.be.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>van Camp</LastName>
<ForeName>John</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Laboratory of Food Chemistry and Human Nutrition, Ghent University, Ghent B-9000, Belgium. John.VanCamp@UGent.be.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bracke</LastName>
<ForeName>Marc</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Laboratory of Experimental Cancer Research, University Hospital Ghent, Ghent B-9000, Belgium. Marc1.Bracke@UGent.be.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Uyttendaele</LastName>
<ForeName>Mieke</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Laboratory of Food Microbiology and Food Preservation, Ghent University, Ghent B-9000, Belgium. Mieke.Uyttendaele@UGent.be.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bačun-Družina</LastName>
<ForeName>Višnja</ForeName>
<Initials>V</Initials>
<AffiliationInfo><Affiliation>Laboratory for Biology and Microbial Genetics, Faculty of Food Technology and Biotechnology, Zagreb University, Zagreb HR-10000, Croatia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cindrić</LastName>
<ForeName>Mario</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Laboratory for System Biomedicine and Centre for Proteomics and Mass Spectrometry, "Ruđer Bošković" Institute, Zagreb HR-10000, Croatia. mcindric@irb.hr.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2014</Year>
<Month>08</Month>
<Day>04</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Switzerland</Country>
<MedlineTA>Toxins (Basel)</MedlineTA>
<NlmUniqueID>101530765</NlmUniqueID>
<ISSNLinking>2072-6651</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D047630">Depsipeptides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004639">Emetics</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C089294">cereulide</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>33X04XA5AT</RegistryNumber>
<NameOfSubstance UI="D019344">Lactic Acid</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001409" MajorTopicYN="N">Bacillus cereus</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018938" MajorTopicYN="N">Caco-2 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D047630" MajorTopicYN="N">Depsipeptides</DescriptorName>
<QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004639" MajorTopicYN="N">Emetics</DescriptorName>
<QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020895" MajorTopicYN="N">Enterocytes</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019344" MajorTopicYN="N">Lactic Acid</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013081" MajorTopicYN="N">Sperm Motility</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D034421" MajorTopicYN="N">Sus scrofa</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year>
<Month>03</Month>
<Day>02</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2014</Year>
<Month>07</Month>
<Day>18</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2014</Year>
<Month>07</Month>
<Day>22</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2014</Year>
<Month>8</Month>
<Day>6</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2014</Year>
<Month>8</Month>
<Day>6</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2015</Year>
<Month>4</Month>
<Day>1</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">25093386</ArticleId>
<ArticleId IdType="pii">toxins6082270</ArticleId>
<ArticleId IdType="doi">10.3390/toxins6082270</ArticleId>
<ArticleId IdType="pmc">PMC4147582</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Toxicol Lett. 2013 Jan 10;216(1):23-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23154127</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lipids Health Dis. 2012;11:169</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23227865</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Biotechnol. 2013 Jan 25;30(2):238-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22683938</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Toxins (Basel). 2013 Apr;5(4):784-820</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23612752</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiol Mol Biol Rev. 2013 Sep;77(3):380-439</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24006470</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Circulation. 2013 Nov 5;128(19):2113-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24014831</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Circulation. 2013 Nov 26;128(22):2364-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24170386</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pharmacol Toxicol Methods. 2014 Jan-Feb;69(1):39-48</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24149024</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Microbiol Methods. 2014 May;100:137-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24632519</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pharm Sci. 1994 Jul;83(7):1006-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7965656</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Chem. 1996 Mar 1;68(5):850-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8779443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 1997 Apr 17;336(16):1142-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9099658</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2011 Apr;77(8):2755-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21357430</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Neurochem. 2007 Dec;103(6):2640-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17953665</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2011 May;11(10):1903-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21462343</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Food Microbiol. 2011 Aug;28(5):1105-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21569959</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Innate Immun. 2011 Feb;17(3):327-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20501516</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Res Toxicol. 2011 Jun 20;24(6):926-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21504230</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Crit Rev Microbiol. 2011 Aug;37(3):188-213</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21417966</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Glia. 2011 Nov;59(11):1672-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21748807</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biol Cell. 2011 Nov;103(11):499-517</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21787361</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2011 Dec;49(12):4379-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22012017</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1997 Oct 3;272(40):25022-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9312109</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Endocrinol Metab. 1997 Oct;82(10):3383-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9329373</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2005 Mar 14;1747(2):151-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15698949</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2005 May;5(7):1914-27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15816005</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2005 Aug;43(8):4277-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16082000</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Z Naturforsch C. 2005 Sep-Oct;60(9-10):799-806</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16320626</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Food Prot. 2006 Apr;69(4):847-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16629028</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Microbiol Methods. 2006 Jun;65(3):525-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16303197</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Med Res Opin. 2006 Jun;22(6):1075-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16846540</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Toxicol. 2006 Sep;80(9):572-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16501953</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Food Prot. 2006 Nov;69(11):2587-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17133800</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Toxicon. 2007 Mar 1;49(3):351-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17156808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Electrophoresis. 1999 Dec;20(18):3551-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10612281</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hum Pathol. 2000 Feb;31(2):149-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10685628</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Artif Organs. 2001 Nov;24(11):807-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11797851</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anticancer Res. 2002 Jul-Aug;22(4):1933-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12174867</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Cell Dev Biol. 2002;18:315-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12142277</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Toxicol In Vitro. 2003 Oct-Dec;17(5-6):737-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14599471</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>In Vitro Cell Dev Biol Anim. 2003 Jul-Aug;39(7):329-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12954076</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Biochim Biophys Sin (Shanghai). 2004 Jun;36(6):419-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15188057</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Microbiol Lett. 2004 Jul 15;236(2):319-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15251214</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Neuroscience. 2004;127(2):443-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15262334</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Appl Microbiol. 2004;97(5):992-1000</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15479414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1970 Aug 15;227(5259):680-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">5432063</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1975 May 25;250(10):4007-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">236308</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol Methods. 1983 Dec 16;65(1-2):55-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6606682</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Toxicol. 1987 Apr;59(6):402-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3606384</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cancer Res. 1988 Apr 1;48(7):1936-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3349466</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gastroenterology. 1989 Mar;96(3):736-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2914637</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Cancer. 1991;27(7):897-900</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1834124</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Food Microbiol. 2007 Feb 28;114(1):92-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17174428</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2006;1(3):1112-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17406391</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2007 Jun;7(11):1757-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17464941</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Endotoxin Res. 2007;13(1):58-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17621547</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lett Appl Microbiol. 2008 May;46(5):536-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18363653</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Toxicon. 2008 May;51(6):1029-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18374382</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cytokine. 2008 Jul;43(1):83-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18501625</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Toxicon. 2009 Aug;54(2):161-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19374914</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Proteomics. 2009 Jun;8(6):1265-77</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19218587</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Free Radic Biol Med. 2010 Feb 15;48(4):519-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19969073</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Food Prot. 2010 Feb;73(2):395-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20132691</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Pediatrics. 2010 Apr;125(4):e951-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20194285</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proteomics. 2010 May;10(9):1831-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20198640</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Toxicol In Vitro. 2010 Oct;24(7):2041-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20732404</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Appl Environ Microbiol. 2010 Nov;76(22):7466-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20889798</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS J. 2010 Sep;277(18):3735-49</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20718861</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Cell Physiol. 2011 Jun;226(6):1531-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20945374</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Cycle. 2011 Dec 1;10(23):4065-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22129993</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Toxicol Lett. 2012 Feb 5;208(3):232-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22138271</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Appl Microbiol. 2012 May;112(5):1042-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22394210</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Toxicon. 2012 Sep 15;60(4):572-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22543189</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Peptides. 2012 Aug;36(2):257-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22659412</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Foodborne Pathog Dis. 2012 Sep;9(9):809-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22891880</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Toxicol In Vitro. 2012 Dec;26(8):1243-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22465559</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Belgique</li>
<li>Croatie</li>
</country>
<region><li>Province de Flandre-Orientale</li>
<li>Région flamande</li>
</region>
<settlement><li>Gand</li>
</settlement>
<orgName><li>Université de Gand</li>
</orgName>
</list>
<tree><country name="Belgique"><region name="Région flamande"><name sortKey="Rajkovic, Andreja" sort="Rajkovic, Andreja" uniqKey="Rajkovic A" first="Andreja" last="Rajkovic">Andreja Rajkovic</name>
</region>
<name sortKey="Bracke, Marc" sort="Bracke, Marc" uniqKey="Bracke M" first="Marc" last="Bracke">Marc Bracke</name>
<name sortKey="Cucu, Tatiana" sort="Cucu, Tatiana" uniqKey="Cucu T" first="Tatiana" last="Cucu">Tatiana Cucu</name>
<name sortKey="De Meulenaer, Bruno" sort="De Meulenaer, Bruno" uniqKey="De Meulenaer B" first="Bruno" last="De Meulenaer">Bruno De Meulenaer</name>
<name sortKey="Grootaert, Charlotte" sort="Grootaert, Charlotte" uniqKey="Grootaert C" first="Charlotte" last="Grootaert">Charlotte Grootaert</name>
<name sortKey="Uyttendaele, Mieke" sort="Uyttendaele, Mieke" uniqKey="Uyttendaele M" first="Mieke" last="Uyttendaele">Mieke Uyttendaele</name>
<name sortKey="Van Camp, John" sort="Van Camp, John" uniqKey="Van Camp J" first="John" last="Van Camp">John Van Camp</name>
</country>
<country name="Croatie"><noRegion><name sortKey="Butorac, Ana" sort="Butorac, Ana" uniqKey="Butorac A" first="Ana" last="Butorac">Ana Butorac</name>
</noRegion>
<name sortKey="Ba Un Druzina, Visnja" sort="Ba Un Druzina, Visnja" uniqKey="Ba Un Druzina V" first="Višnja" last="Ba Un-Družina">Višnja Ba Un-Družina</name>
<name sortKey="Cindri, Mario" sort="Cindri, Mario" uniqKey="Cindri M" first="Mario" last="Cindri">Mario Cindri</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/DetoxFungiV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001458 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001458 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    DetoxFungiV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:25093386
   |texte=   Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:25093386" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a DetoxFungiV1

This area was generated with Dilib version V0.6.38.
Data generation: Fri Nov 20 16:09:04 2020. Site generation: Fri Nov 20 16:15:24 2020

	Serveur d'exploration sur la détoxication des champignons
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la détoxication des champignons

Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells.

Sub-emetic toxicity of Bacillus cereus toxin cereulide on cultured human enterocyte-like Caco-2 cells.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki